RFC4 Polyklonaler Antikörper

RFC4 Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10806-1-AP

Synonyme

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C subunit 4, RF C 37 kDa subunit, RFC37, RFC4

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 10806-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 10806-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10806-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10806-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10806-1-AP (RFC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 10806-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 10806-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RFC4 antibody (10806-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 10806-1-AP

  Immunofluorescent analysis of HepG2 cells, using RFC4 antibody 10806-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 10806-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug RFC4 antibody (10806-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, LNCaP-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF: U2OS-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10806-1-AP bindet in WB, IF, FC, ELISA RFC4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFC4 fusion protein Ag1234
• Vollständiger Name: replication factor C (activator 1) 4, 37kDa
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37-40 kDa
• GenBank-Zugangsnummer: BC017452
• Gene symbol: RFC4
• Gene ID (NCBI): 5984
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); The upregulated expression of RFC4 and GMPS mediated by DNA copy number alteration is associated with the early diagnosis and immune escape of ESCC based on a bioinformatic analysis.; Authors - Jing Wang
mouse	WB	Cell Rep; Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.; Authors - Stephen Gray
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang