RFC5 Polyklonaler Antikörper

RFC5 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10385-1-AP

Synonyme

A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10385-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10385-1-AP (RFC5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10385-1-AP

  IP result of anti-RFC5 (IP:10385-1-AP, 4ug; Detection:10385-1-AP 1:300) with HeLa cells lysate 3000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10385-1-AP bindet in WB, IP, ELISA RFC5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFC5 fusion protein Ag0584
• Vollständiger Name: replication factor C (activator 1) 5, 36.5kDa
• Berechnetes Molekulargewicht: 36 aa, 5 kDa
• GenBank-Zugangsnummer: BC001866
• Gene symbol: RFC5
• Gene ID (NCBI): 5985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C(RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. Replication factor C5 (RFC5, synonyms: RFC36, MGC1155) is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA and form a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

Publikationen

Species	Application	Title
human	WB	bioRxiv; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination; Authors - Yi-Chang Wang
human	WB	Cell Rep; Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination; Authors - Yi-Chang Wang