RFTN1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RFTN1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24289-1-AP

Synonyme

Raftlin, PIG9, PIB10, MIG2, KIAA0084

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  WB analysis of HeLa using 24289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 24289-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 24289-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24289-1-AP bindet in WB, IHC, IF, IP, ELISA RFTN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFTN1 fusion protein Ag17264
• Vollständiger Name: raftlin, lipid raft linker 1
• Berechnetes Molekulargewicht: 578 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC100667
• Gene symbol: RFTN1
• Gene ID (NCBI): 23180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).

Publikationen

Species	Application	Title
human	IF,IP	Angiogenesis; Raftlin is recruited by neuropilin-1 to the activated VEGFR2 complex to control proangiogenic signaling.; Authors - Asha L Bayliss
human,mouse	WB	Pathol Res Pract; RFTN1 facilitates gastric cancer progression by modulating AKT/p38 signaling pathways.; Authors - Chaowei Deng ; KD Validated
human	WB	Alzheimers Dement; Amyloid futures in the expanding pathology of brain aging and dementia; Authors - Max A Thorwald