RFTN1 Polyklonaler Antikörper

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• KD/KO Validated

RFTN1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24289-1-PBS

Synonyme

Raftlin, PIG9, PIB10, MIG2, KIAA0084

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• 

  WB analysis of HeLa using 24289-1-AP (same clone as 24289-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 24289-1-AP (same clone as 24289-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 24289-1-AP (same clone as 24289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24289-1-PBS bindet in WB, IHC, Indirect ELISA RFTN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFTN1 fusion protein Ag17264
• Vollständiger Name: raftlin, lipid raft linker 1
• Berechnetes Molekulargewicht: 578 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC100667
• Gene symbol: RFTN1
• Gene ID (NCBI): 23180
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).