RFWD3 Polyklonaler Antikörper

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RFWD3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19893-1-PBS

Synonyme

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  WB analysis of mouse lung using 19893-1-AP (same clone as 19893-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 19893-1-AP (RFWD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 19893-1-AP (RFWD3 antibody) at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 19893-1-AP (same clone as 19893-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 19893-1-AP (RFWD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 19893-1-AP (same clone as 19893-1-PBS)

  Immunofluorescent analysis of 293 cells, using RFWD3 antibody 19893-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19893-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19893-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RFWD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RFWD3 fusion protein Ag13728
• Vollständiger Name: ring finger and WD repeat domain 3
• Berechnetes Molekulargewicht: 774 aa, 85 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC002574
• Gene symbol: RFWD3
• Gene ID (NCBI): 55159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFWD3, also named as RNF201, is a E3 ubiquitin-protein ligase that mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. RFWD3 may act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. In response to ionizing radiation, It interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53.(PMID: 20173098 ) From BLAST result, this antibody is specific to RFWD3. 45kd and 55kd maybe splicesome of RFWD3.