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RFX1 Monoklonaler Antikörper

RFX1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G10H6

Kat-Nr. : 68057-1-PBS

Synonyme

RFX, EF-C, EF C, 1G10H6

Filter:
  • Western Blot (WB) analysis of various lysates using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis using 68057-1-Ig (same clone as 68057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U2OS cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of U2OS using 68057-1-Ig (same clone as 68057-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of HeLa using 68057-1-Ig (same clone as 68057-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using RFX1 Monoclonal antibody (68057-1-Ig)

    WB analysis of NIH/3T3 using 68057-1-Ig (same clone as 68057-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68057-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68057-1-PBS bindet in WB, Indirect ELISA RFX1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RFX1 fusion protein Ag25442
Vollständiger Name regulatory factor X, 1 (influences HLA class II expression)
Berechnetes Molekulargewicht 105 kDa
Beobachtetes Molekulargewicht135 kDa
GenBank-ZugangsnummerBC049826
Gene symbol RFX1
Gene ID (NCBI) 5989
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).