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RFX1 Monoklonaler Antikörper

RFX1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G10H6

Kat-Nr. : CL488-68057

Synonyme

RFX, EF-C, EF C, 1G10H6

Filter:
  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated RFX1 Monoclonal anti (CL488-68057)

    FC experiment of Jurkat using CL488-68057

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human RFX1 (CL488-68057, Clone:1G10H6) (red), or 0.2 ug x. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Jurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-68057 bindet in FC (Intra) RFX1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RFX1 fusion protein Ag25442
Vollständiger Name regulatory factor X, 1 (influences HLA class II expression)
Berechnetes Molekulargewicht 105 kDa
Beobachtetes Molekulargewicht135 kDa
GenBank-ZugangsnummerBC049826
Gene symbol RFX1
Gene ID (NCBI) 5989
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).