RGMA Polyklonaler Antikörper

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• KD/KO Validated

RGMA Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12387-1-AP

Synonyme

Repulsive guidance molecule A, RGM, RGM domain family member A, RGM domain family, member A, RGMA

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Galerie der Validierungsdaten

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  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 12387-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 12387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12387-1-AP (RGMA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 12387-1-AP

  IP Result of anti-RGMA (IP:12387-1-AP, 4ug; Detection:12387-1-AP 1:400) with mouse testis tissue lysate 4000ug.

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  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 12387-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12387-1-AP (RGMA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Caco-2-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe, Maushodengewebe, MOLT-4-Zellen, Rattenhirngewebe, U2OS-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12387-1-AP bindet in WB, IP, IHC, CoIP, ELISA RGMA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGMA fusion protein Ag3058
• Vollständiger Name: RGM domain family, member A
• Berechnetes Molekulargewicht: 450 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC015886
• Gene symbol: RGMA
• Gene ID (NCBI): 56963
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGMA, also named as RGM, is a member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. It regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. RGMA binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. RGMA is a molecular target for neuroprotection in retinal pathologies(PMID: 20457227). the frequent genetic and epigenetic inactivation of RGMA in CRCs and adenomas along with its in vitro function collectively support its role as a tumor suppressor in colon cells(PMID:19303019 ). There're some different MW in WB detection, 30 kDa mature form, 47-50 kDa isoforms and 60 kDa Glycosylated form.

Publikationen

Species	Application	Title
rat	WB	Brain Res Bull; Adenoviral Vector-Induced Silencing of RGMa Attenuates Blood-Brain Barrier Dysfunction in a Rat Model of MCAO/Reperfusion.; Authors - Min Li ; KD Validated
mouse	CoIP	Sci Adv; RGMa collapses the neuronal actin barrier against disease-implicated protein and exacerbates ALS; Authors - Mikito Shimizu