RGR Polyklonaler Antikörper

RGR Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11904-1-PBS

Synonyme

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  WB analysis of human heart using 11904-1-AP (same clone as 11904-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11904-1-AP (RGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 11904-1-AP (same clone as 11904-1-PBS)

  IP result of anti-RGR (IP:11904-1-AP, 4ug; Detection:11904-1-AP 1:300) with PC-3 cells lysate 4000ug. This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse retina using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse retina using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse retina tissue slide using 11904-1-AP (RGR antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11904-1-AP (same clone as 11904-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11904-1-AP (RGR antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11904-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11904-1-PBS bindet in WB, IHC, IP, Indirect ELISA RGR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGR fusion protein Ag2552
• Vollständiger Name: retinal G protein coupled receptor
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 26 kDa
• GenBank-Zugangsnummer: BC008094
• Gene symbol: RGR
• Gene ID (NCBI): 5995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGR is a G protein-coupled receptor preferentially expressed at high levels in the retinal pigment epithelium (RPE) and Mueller cells of the neural retina. Like other opsins which bind retinaldehyde, it contains a conserved lysine residue in the seventh transmembrane domain. RGR acts as a photoisomerase to catalyze the conversion of all-trans-retinal to 11-cis-retinal. The reverse isomerization occurs with rhodopsin in retinal photoreceptor cells. The gene of PGR may be associated with autosomal recessive and autosomal dominant retinitis pigmentosa (arRP and adRP, respectively). Alternative splicing results in multiple transcript variants encoding different isoforms.