• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RGS12 Polyklonaler Antikörper

RGS12 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29803-1-PBS

Synonyme

Regulator of G-protein signaling 12

Filter:
  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RGS12 Polyclonal antibody (29803-1-AP)

    WB analysis using 29803-1-AP (same clone as 29803-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29803-1-AP (RGS12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using RGS12 Polyclonal antibody (29803-1-AP)

    IHC staining of rat cerebellum using 29803-1-AP (same clone as 29803-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 29803-1-AP (RGS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using RGS12 Polyclonal antibody (29803-1-AP)

    IF Staining of PC-3 using 29803-1-AP (same clone as 29803-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using RGS12 antibody (29803-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29803-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29803-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RGS12 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RGS12 fusion protein Ag30731
Vollständiger Name regulator of G-protein signaling 12
Berechnetes Molekulargewicht 156 kDa
Beobachtetes Molekulargewicht140-150 kDa
GenBank-ZugangsnummerNM_198229
Gene symbol RGS12
Gene ID (NCBI) 6002
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Regulator of G-protein signaling 12 (RGS12) regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. This antibody can be detected as 140-150 kDa.