RGS12 Rekombinanter Antikörper

RGS12 Rekombinant Antikörper für WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242530G11

Kat-Nr. : 85057-2-PBS

Synonyme

242530G11

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• 

  WB analysis using 85057-2-RR (same clone as 85057-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85057-2-RR (RGS12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP01790-2

  Sandwich ELISA standard curve of MP01790-2, Human RGS12 Recombinant Matched Antibody Pair - PBS only. 85057-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag30731. 85057-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Sample test of MP01790-2

  The mean RGS12 concentration was determined to be 18,450.2 pg/mL in A431 cell extract based on a 1.3 mg/mL extract load, 6,327.2 pg/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 42,390.5 pg/mL in SH-SY5Y cell extract based on a 3.2 mg/mL extract load.

• 

  IF Staining of PC-3 using 85057-2-RR (same clone as 85057-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using RGS12 antibody (85057-2-RR, Clone: 242530G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85057-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01790-2

  Cytometric bead array standard curve of MP01790-2, RGS12 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85057-3-PBS. Detection antibody: 85057-2-PBS. Standard: Ag30731. Range: 0.625-40 ng/mL.

• 

  Affinity and Kinetic Characterization of 85057-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85057-2-RR against Human RGS12 were performed. The affinity constant is 0.611 nM.

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Geprüfte Anwendungen

Produktinformation

85057-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test RGS12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RGS12 fusion protein Ag30731
• Vollständiger Name: regulator of G-protein signaling 12
• Berechnetes Molekulargewicht: 156 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: NM_198229
• Gene symbol: RGS12
• Gene ID (NCBI): 6002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.