RGS3 Monoklonaler Antikörper

RGS3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1D6A3

Kat-Nr. : 66790-1-Ig

Synonyme

C2PA, FLJ20370, FLJ31516, FLJ90496, PDZ RGS3, RGP3, RGS3

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  WB analysis of Jurkat using 66790-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66790-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66790-1-Ig (RGS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MCF-7 using 66790-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RGS3 antibody (66790-1-Ig, Clone: 1D6A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

66790-1-Ig bindet in WB, ELISA RGS3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RGS3 fusion protein Ag3177
• Vollständiger Name: regulator of G-protein signaling 3
• Berechnetes Molekulargewicht: 1198 aa, 132 kDa
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: BC018072
• Gene symbol: RGS3
• Gene ID (NCBI): 5998
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS3 belongs to a family of proteins which share the core regulator of G-protein signalling (RGS) domain and function as GTPase-activating proteins (GAPs) for the α subunit of heterotrimeric G-proteins. RGS3 has been reported to inhibit the Gai-and Gaq-mediated signaling pathways in the cardiovascular system, including those acting via the cardiovascular signaling molecules angiotensin II (Ang II), sphingosine 1-phosphate and endothelin-1. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear.

Publikationen

Species	Application	Title
mouse	WB	Transl Cancer Res; Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1; Authors - Xiao Hu
human	WB	Stem Cell Res Ther; Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy; Authors - Xuehui Fan