RGS5 Polyklonaler Antikörper

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• KD/KO Validated

RGS5 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11590-1-PBS

Synonyme

Regulator of G-protein signaling 5

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• 

  WB analysis using 11590-1-AP (same clone as 11590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11590-1-AP (same clone as 11590-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11590-1-AP (same clone as 11590-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse skeletal muscle using 11590-1-AP (same clone as 11590-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11590-1-AP (same clone as 11590-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 11590-1-AP (same clone as 11590-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 11590-1-AP (same clone as 11590-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11590-1-AP (RGS5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 11590-1-AP (same clone as 11590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 11590-1-AP (same clone as 11590-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11590-1-AP (RGS5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11590-1-AP (same clone as 11590-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RGS5 antibody (11590-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 11590-1-AP (same clone as 11590-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RGS5 (11590-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11590-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11590-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA RGS5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGS5 fusion protein Ag2196
• Vollständiger Name: regulator of G-protein signaling 5
• Berechnetes Molekulargewicht: 181 aa, 21 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC030059
• Gene symbol: RGS5
• Gene ID (NCBI): 8490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kd band recognized by this antibody is a RGS5-RGS5 dimer. (Pubmed:17762159)