RGS9 Polyklonaler Antikörper

RGS9 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17970-1-AP

Synonyme

PERRS, RGS9, RGS9L

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 17970-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17970-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17970-1-AP (RGS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

17970-1-AP bindet in WB, ELISA RGS9 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RGS9 fusion protein Ag11976
• Vollständiger Name: regulator of G-protein signaling 9
• Berechnetes Molekulargewicht: 622 aa, 71 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 70-75 kDa
• GenBank-Zugangsnummer: BC022504
• Gene symbol: RGS9
• Gene ID (NCBI): 8787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RGS9 is a member of the RGS family of signaling proteins that suppress the activity of G proteins by promoting their deactivation. There are two splice isoforms of RGS9 with quite different properties and patterns of expression. RGS9-1 is mainly found in the eye and is involved in regulation of phototransduction in rod and cone cells of the retina, while RGS9-2 is is expressed specifically in the striatum, a brain region involved in controlling movement, motivation, mood and addition. This antibody got about 58kD in the mouse eye and 70kD in the mouse brain in western blotting, both in line with their expected masses. Defects in RGS9 are a cause of prolonged electroretinal response suppression (PERRS).

Publikationen

Species	Application	Title
human	WB	Genes (Basel); Extended Phenotyping and Functional Validation Facilitate Diagnosis of a Complex Patient Harboring Genetic Variants in MCCC1 and GNB5 Causing Overlapping Phenotypes.; Authors - Zhuo Shao