RHBDF2 Polyklonaler Antikörper

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• KD/KO Validated

RHBDF2 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 23181-1-AP

Synonyme

Rhomboid family member 2, Rhomboid 5 homolog 2, RHBDL6, RHBDL5, iRhom2

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  IHC staining of human colon cancer using 23181-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23181-1-AP (RHBDF2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 23181-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23181-1-AP (RHBDF2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 23181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RHBDF2 antibody (23181-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 23181-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RHBDF2 antibody (23181-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of A431 using 23181-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using RHBDF2 antibody (23181-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IP: See 1 publications below

Produktinformation

23181-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RHBDF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHBDF2 fusion protein Ag19710
• Vollständiger Name: rhomboid 5 homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 856 aa, 97 kDa
• GenBank-Zugangsnummer: BC016034
• Gene symbol: RHBDF2
• Gene ID (NCBI): 79651
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The gene for rhomboid 5 homolog 2 (RHBDF2) is a protein-coding gene that encodes rhombus protease, iRhom2, which participates in important biological processes. RHBDF2 is significantly upregulated in tumors and has pro-tumor effects.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Cell Int; RHBDF2 gene functions are correlated to facilitated renal clear cell carcinoma progression.; Authors - Lei Wang ; KD Validated
human	WB,IHC	FEBS Open Bio; RHBDF2 is correlated with immune infiltrates in hepatocellular carcinoma and may have potential as a biomarker; Authors - Hanjuan Gong
human	WB,IHC	Biotechnol Genet Eng Rev; The prognostic value of RHBDF2 in Pan-Cancer, and its correlation with cell Adhesion of Hepatocellular Carcinoma; Authors - Hanjuan Gong
monkey	WB	J Virol; The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1; Authors - Feifei Diao
pig	WB,IP	Vet Microbiol; Porcine reproductive and respiratory syndrome virus infection activates ADAM17 to induce inflammatory responses; Authors - Jiao Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (02-20-2019)

This antibody cannot be used for WB blot. It gives multiple bands, and the band around the expected molecular weight was still there in iRhom2 (another name for Rhbdf2) KO or KD samples.

• Applications: Western Blot,