RHEB Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RHEB Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 15924-1-AP

Synonyme

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

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Galerie der Validierungsdaten

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  WB analysis using 15924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 15924-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 15924-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15924-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of 3T3-L1 using 15924-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15924-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHEB antibody (15924-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, 3T3-L1-Zellen, fetales humanes Hirngewebe, HeLa-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Pankreaskarzinomgewebe, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

15924-1-AP bindet in WB, IHC, IF, ELISA RHEB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHEB fusion protein Ag8747
• Vollständiger Name: Ras homolog enriched in brain
• Berechnetes Molekulargewicht: 184 aa, 20 kDa
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC016155
• Gene symbol: RHEB
• Gene ID (NCBI): 6009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Publikationen

Species	Application	Title
rat	WB	Cell Metab; Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.; Authors - Noriaki Shimizu
mouse	IHC	PLoS Biol; RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.; Authors - Martina Proietti Onori
mouse	IF	Metabolism; Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.; Authors - Yan Yang ; KO Validated
human		Front Cell Dev Biol; PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.; Authors - Qingqiong Luo
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin
human	WB	Exp Mol Med; A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II.; Authors - Shanshan Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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