RHEB Monoklonaler Antikörper

RHEB Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F9H8

Kat-Nr. : 66714-1-PBS

Synonyme

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

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  WB analysis of fetal human brain using 66714-1-Ig (same clone as 66714-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66714-1-Ig (same clone as 66714-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66714-1-Ig (same clone as 66714-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 66714-1-Ig (same clone as 66714-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 66714-1-Ig (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66714-1-Ig (RHEB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  IF Staining of human pancreas cancer using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pancreas using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 66714-1-Ig (same clone as 66714-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (66714-1-Ig, Clone: 2F9H8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66714-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66714-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA RHEB und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RHEB fusion protein Ag8887
• Vollständiger Name: Ras homolog enriched in brain
• Berechnetes Molekulargewicht: 184 aa, 20 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC016155
• Gene symbol: RHEB
• Gene ID (NCBI): 6009
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.