RHOA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RHOA Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(174)

Kat-Nr. : 10749-1-AP

Synonyme

ARH12, ARHA, RHO12, EC:3.6.5.2, h12

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  WB analysis using 10749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10749-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 10749-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 10749-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 10749-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10749-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10749-1-AP (RHOA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10749-1-AP

  IP result of anti-RHOA (IP:10749-1-AP, 4ug; Detection:10749-1-AP 1:4000) with mouse brain tissue lysate 2100 ug.

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  IF Staining of HeLa using 10749-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHOA antibody (10749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of K-562 using 10749-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using RHOA antibody (10749-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, fetales humanes Hirngewebe, HL-60-Zellen, HUVEC-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 159 publications below
• IHC: See 15 publications below
• IF: See 18 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10749-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RHOA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHOA fusion protein Ag1141
• Vollständiger Name: ras homolog gene family, member A
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC005976
• Gene symbol: RHOA
• Gene ID (NCBI): 387
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

What is the function of RhoA?

Ras homolog gene family, member A (RhoA) is a small GTPase that is involved in cytoskeleton organization.¹ It is important in actomyosin contractility, as when RhoA binds to GTP it initiates a cascade of events mediating stress fiber or contractile ring formation, where actin bundles crosslink with other proteins. It is also associated with actin polymerization, making RhoA important in membrane ruffling and cell motility.² The role of RhoA is essential in cell migration and adhesion.

 What is the cellular localization of RhoA?

In motile cells, a leading edge stretches ahead, stabilizes, the cytoskeleton is reorganized, and the tail of the cell retracts. The role of RhoA in contracting actomyosin has linked RhoA with the tail retraction in this process, but studies into the spatiotemporal kinetics of GTPases have revealed that RhoA is also located at the leading edge of migrating cells.³ This highlights the dual roles of this protein in actin contractility and polymerization.

 What is the role of RhoA in disease?

Though it is not an oncogene, the role of RhoA in adhesion and migration has implicated this protein in cancer biology. Expression of RhoA has been found to be higher in malignant tumors compared to benign tumors or in non-tumor tissue, and more invasive tumors have been found to overexpress RhoA.⁴ This protein is also implicated in neurodegenerative diseases and aging due to its role in axon guidance, with abnormal localization of RhoA associated with Alzheimer's and Parkinson's pathology.^5,6 

1.     Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 70, 389-399 (1992).

2.     21.   Kurokawa, K. & Matsuda, M. Localized RhoA Activation as a Requirement for the Induction of Membrane Ruffling. Mol. Biol. Cell 16, 4294-4303 (2005).

3.     22.   Struckhoff, A. P., Rana, M. K. & Worthylake, R. A. RhoA can lead the way in tumor cell invasion and metastasis. Front. Biosci. (Landmark Ed. 16, 1915-26 (2011).

4.     23.   Mcglasson, S. et al. Rare variants of the 3'-5' DNA exonuclease in early onset TREX1 small vessel stroke [version 1; referees: awaiting peer review]. doi:10.12688/wellcomeopenres.12631.1

5.     24.   Huesa, G. et al. Altered Distribution of RhoA in Alzheimer's Disease and AβPP Overexpressing Mice. J. Alzheimer's Dis. 19, 37-56 (2010).

6.     25.   Hynds, D. L. Subcellular localization of Rho GTPases: implications for axon regeneration. Neural Regen. Res. 10, 1032-3 (2015).

Publikationen

Species	Application	Title
Human	WB	Cancer Cell; Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis; Authors - Wei Zhou
human	IF	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
mouse	WB	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	IF	Biomater Res; Near-Infrared Light-Controlled Dynamic Hydrogel for Modulating Mechanosensitive Ion Channels in 3-Dimensional Environment; Authors - Xiaoning Liu
human	WB	Acta Biomater; Adipose-derived stem cells with miR-150-5p inhibition laden in hydroxyapatite/tricalcium phosphate ceramic powders promote osteogenesis via regulating Notch3 and activating FAK/ERK and RhoA; Authors - Fanglin Wang
mouse	WB	Autophagy; m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.; Authors - Yabing Chen