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RHOA Monoklonaler Antikörper

RHOA Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B3D7

Kat-Nr. : 66733-1-PBS

Synonyme

ARH12, ARHA, RHO12, 1B3D7, EC:3.6.5.2

Filter:
  • Western Blot (WB) analysis of various lysates using RHOA Monoclonal antibody (66733-1-Ig)

    WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOA Monoclonal antibody (66733-1-Ig)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOA Monoclonal antibody (66733-1-Ig)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOA Monoclonal antibody (66733-1-Ig)

    IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66733-1-PBS bindet in WB, IHC, IF/ICC, ELISA RHOA und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RHOA fusion protein Ag1141
Vollständiger Name ras homolog gene family, member A
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.