• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

RHOA Rekombinanter Antikörper

RHOA Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240880E5

Kat-Nr. : 82760-2-PBS

Synonyme

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Filter:
  • Western Blot (WB) analysis of various lysates using RHOA Recombinant antibody (82760-2-RR)

    WB analysis using 82760-2-RR (same clone as 82760-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82760-2-RR (RHOA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RHOA Recombinant antibody (82760-2-RR)

    WB analysis of HEK-293 using 82760-2-RR (same clone as 82760-2-PBS)

    WB result of RHOA antibody (82760-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RHOA transfected HEK-293 cells. This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOA Recombinant antibody (82760-2-RR)

    IF Staining of HeLa using 82760-2-RR (same clone as 82760-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOA antibody (82760-2-RR, Clone: 240880E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82760-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82760-2-RR

    Affinity and Kinetic Characterization of 82760-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82760-2-RR against Human RHOA were performed. The affinity constant is 1.18 nM.

Geprüfte Anwendungen

Produktinformation

82760-2-PBS bindet in WB, IF/ICC, Indirect ELISA RHOA und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RHOA fusion protein Ag1141
Vollständiger Name ras homolog gene family, member A
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 22 kDa
GenBank-ZugangsnummerBC005976
Gene symbol RHOA
Gene ID (NCBI) 387
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.