RHOB Polyklonaler Antikörper

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• KD/KO Validated

RHOB Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(29)

Kat-Nr. : 14326-1-AP

Synonyme

ARH6, ARHB, h6, Rho cDNA clone 6, Rho-related GTP-binding protein RhoB

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  WB analysis using 14326-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14326-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14326-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14326-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14326-1-AP (RHOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 3ug; Detection:14326-1-AP 1:2000) with HeLa cells lysate 2800ug.

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  IP experiment of mouse brain using 14326-1-AP

  IP result of anti-RHOB (IP:14326-1-AP, 4ug; Detection:14326-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 14326-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 14326-1-AP (RHOB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HeLa-Zellen, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

14326-1-AP bindet in WB, IHC, IF, IP, ELISA RHOB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHOB fusion protein Ag5593
• Vollständiger Name: ras homolog gene family, member B
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC066954
• Gene symbol: RHOB
• Gene ID (NCBI): 388
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rho-related GTP-binding protein RhoB (RHOB) is also named ARH6, ARHB and Rho cDNA clone 6 (h6). RhoB was the first member of the Rho family to be implicated in endosomal trafficking. One study showed that RhoB localizes and activates its downstream target, serine/threonine kinase (PRK1), on endosomes, and acts through this signaling pathway to disrupt the trafficking of internalized EGF receptor from endosomes to a prelysosomal compartment (PMID: 29385717). RhoB is known to be part of the immediate early genetic response to epidermal growth factor, transforming growth factor β, Src activation, or genotoxic stress (PMID:9545335, PMID: 10679283). RhoB was shown to have potential implications for EGF signaling by targeting the activated EGF receptors to the lysosome, which represents an "off-switch" for mitogenic signals (PMID: 1050858). RhoB was also demonstrated to exert a negative regulatory influence on TGF-β-induced transcriptional activation (PMID:9545335). The activity of the RhoB promoter was stimulated by genotoxic treatments indicating its role in the cellular response to DNA damage (PMID:9388198).

Publikationen

Species	Application	Title
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB,IF	Nat Commun; ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage.; Authors - Mingdong Liu ; KO Validated
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Mol Cancer Ther; Expression signatures of the lipid-based Akt inhibitors phosphatidylinositol ether lipid analogues in NSCLC cells.; Authors - Zhang Chunyu C ; KD Validated
rat	WB	Sci Rep; MicroRNA-223 Attenuates Hypoxia-induced Vascular Remodeling by Targeting RhoB/MLC2 in Pulmonary Arterial Smooth Muscle Cells.; Authors - Yan Zeng
mouse	WB	Invest Ophthalmol Vis Sci; Epigenetic ALYREF/UHRF1/RHOB Axis in Corneal Wound Healing and Implications for Epithelial Tumorigenesis; Authors - Hua Zhang