• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RHOB Rekombinanter Antikörper

RHOB Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251662A8

Kat-Nr. : 86663-3-PBS

Synonyme

ARH6, ARHB, h6, Rho cDNA clone 6, Rho-related GTP-binding protein RhoB

Filter:
  • Western Blot (WB) analysis of various lysates using RHOB Recombinant monoclonal antibody (86663-3-RR)

    WB analysis using 86663-3-RR (same clone as 86663-3-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 86663-3-RR (RHOB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86663-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86663-3-RR

    Affinity and Kinetic Characterization of 86663-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86663-3-RR against Human RHOB were performed. The affinity constant is 2.59 nM.

Geprüfte Anwendungen

Produktinformation

86663-3-PBS bindet in WB, Indirect ELISA RHOB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RHOB fusion protein Ag5593
Vollständiger Name ras homolog gene family, member B
Berechnetes Molekulargewicht 22 kDa
Beobachtetes Molekulargewicht 22-26 kDa
GenBank-ZugangsnummerBC066954
Gene symbol RHOB
Gene ID (NCBI) 388
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rho-related GTP-binding protein RhoB (RHOB) is also named ARH6, ARHB and Rho cDNA clone 6 (h6). RhoB was the first member of the Rho family to be implicated in endosomal trafficking. One study showed that RhoB localizes and activates its downstream target, serine/threonine kinase (PRK1), on endosomes, and acts through this signaling pathway to disrupt the trafficking of internalized EGF receptor from endosomes to a prelysosomal compartment (PMID: 29385717). RhoB is known to be part of the immediate early genetic response to epidermal growth factor, transforming growth factor β, Src activation, or genotoxic stress (PMID:9545335, PMID: 10679283). RhoB was shown to have potential implications for EGF signaling by targeting the activated EGF receptors to the lysosome, which represents an "off-switch" for mitogenic signals (PMID: 1050858). RhoB was also demonstrated to exert a negative regulatory influence on TGF-β-induced transcriptional activation (PMID:9545335). The activity of the RhoB promoter was stimulated by genotoxic treatments indicating its role in the cellular response to DNA damage (PMID:9388198).