RHOBTB1 Rekombinanter Antikörper

RHOBTB1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240253F10

Kat-Nr. : 83342-1-RR

Synonyme

KIAA0740, RHOBTB1

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  WB analysis using 83342-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83342-1-RR (RHOBTB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83342-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RHOBTB1 antibody (83342-1-RR, Clone: 240253F10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 83342-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83342-1-RR against Human RHOBTB1 were performed. The affinity constant is 0.502 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HT-29-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83342-1-RR bindet in WB, ELISA RHOBTB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RHOBTB1 fusion protein Ag3365
• Vollständiger Name: Rho-related BTB domain containing 1
• Berechnetes Molekulargewicht: 696 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC032848
• Gene symbol: RHOBTB1
• Gene ID (NCBI): 9886
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rho-related BTB domain containing protein 1 (RhoBTB1) is also named as KIAA0740. RhoBTB1 is an atypical Rho GTPase with two BTB domains in addition to its Rho domain. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its association with Cullin3, a scaffold for ubiquitin ligases (PMID: 31431478). RHOBTB1 is a novel PPARγ gene target in vascular smooth muscle cells that mediates the protective effect of PPARγ by serving as a substrate adaptor between the Cullin-3 RING ubiquitin ligase and phosphodiesterase 5, thus restraining its activity through ubiquitination and proteasomal degradation (PMID: 31789920).