RHOBTB3 Monoklonaler Antikörper

RHOBTB3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2E3

Kat-Nr. : 67502-1-Ig

Synonyme

KIAA0878, RHOBTB3

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 67502-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67502-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 67502-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67502-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67502-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67502-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67502-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Hausschwein-Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67502-1-Ig bindet in WB, IHC, IF, ELISA RHOBTB3 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RHOBTB3 fusion protein Ag5782
• Vollständiger Name: Rho-related BTB domain containing 3
• Berechnetes Molekulargewicht: 611 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC041337
• Gene symbol: RHOBTB3
• Gene ID (NCBI): 22836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.