RHOBTB3 Monoklonaler Antikörper

RHOBTB3 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1H2E3

Kat-Nr. : 67502-1-Ig

Synonyme

1H2E3, EC:3.6.1.-, KIAA0878, Rho-related BTB domain-containing protein 3

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  WB analysis of K-562 using 67502-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67502-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67502-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 67502-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67502-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67502-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat adrenal gland using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 67502-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, Hausschwein-Hirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe, Ratten-Nebennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67502-1-Ig bindet in WB, IHC, ELISA RHOBTB3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RHOBTB3 fusion protein Ag5782
• Vollständiger Name: Rho-related BTB domain containing 3
• Berechnetes Molekulargewicht: 611 aa, 69 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC041337
• Gene symbol: RHOBTB3
• Gene ID (NCBI): 22836
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.