RHOF Polyklonaler Antikörper

RHOF Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12290-1-AP

Synonyme

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

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  WB analysis using 12290-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12290-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12290-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 12290-1-AP

  IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12290-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: COLO 320-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12290-1-AP bindet in WB, IHC, IF, IP, ELISA RHOF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHOF fusion protein Ag2943
• Vollständiger Name: ras homolog gene family, member F (in filopodia)
• Berechnetes Molekulargewicht: 211 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC018208
• Gene symbol: RHOF
• Gene ID (NCBI): 54509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression; Authors - Michiru Nishita
human	WB	Int J Mol Sci; An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer; Authors - Yongcheng Su
human,mouse	WB,IHC,IF	iScience; RHOF activation of AKT/β-catenin signaling pathway drives acute myeloid leukemia progression and chemotherapy resistance; Authors - Xin Wen ; KD Validated
human	IHC	Virchows Arch; High expression of RHOF is an effective diagnostic marker and a potential prognostic indicator for primary mediastinal large B-cell lymphoma; Authors - Xinyi Zhu
human	WB	EPMA J; Deciphering key roles of B cells in prognostication and tailored therapeutic strategies for lung adenocarcinoma: a multi-omics and machine learning approach towards predictive, preventive, and personalized treatment strategies; Authors - Jinjin Zhang