RHOQ/TC10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RHOQ/TC10 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17805-1-AP

Synonyme

RHOQ, TC10, ARHQ, Ras like protein TC10, RASL7A

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  WB analysis using 17805-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17805-1-AP (RHOQ/TC10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17805-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17805-1-AP (TC10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 17805-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17805-1-AP (RHOQ/TC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 17805-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17805-1-AP (RHOQ/TC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17805-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17805-1-AP (RHOQ/TC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

17805-1-AP bindet in WB, IHC, IF, ELISA RHOQ/TC10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RHOQ/TC10 fusion protein Ag12078
• Vollständiger Name: ras homolog gene family, member Q
• Berechnetes Molekulargewicht: 205 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC070485
• Gene symbol: RHOQ/TC10
• Gene ID (NCBI): 23433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Immunol; The Small Rho GTPase TC10 Modulates B Cell Immune Responses.; Authors - Marianne Burbage ; KO Validated
mouse	WB	J Neurochem; TC10, a Rho family GTPase, is required for efficient axon regeneration in a neuron-autonomous manner.; Authors - Shingo Koinuma ; KO Validated
rat		Genes Cells; cAMP-induced activation of protein kinase A and p190B RhoGAP mediates down-regulation of TC10 activity at the plasma membrane and neurite outgrowth.; Authors - Shingo Koinuma
human	IF	Biophys Rep (N Y); TC10 differently controls the dynamics of Exo70 in growth cones of cortical and hippocampal neurons; Authors - Hiteshika Gosain