RIAM Monoklonaler Antikörper

RIAM Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F8A2

Kat-Nr. : 67143-1-PBS

Synonyme

APBB1 interacting protein 1, APBB1IP, APBB1IP,RIAM, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM, RIAM,APBB1IP

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  WB analysis using 67143-1-Ig (same clone as 67143-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 67143-1-Ig (same clone as 67143-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67143-1-Ig (same clone as 67143-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  WB analysis of HL-60 using 67143-1-Ig (same clone as 67143-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67143-1-Ig (RIAM,APBB1IP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67143-1-Ig (same clone as 67143-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using RIAM,APBB1IP antibody (67143-1-Ig, Clone: 2F8A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67143-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67143-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA RIAM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RIAM fusion protein Ag4602
• Vollständiger Name: amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein
• Berechnetes Molekulargewicht: 666 aa, 73 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC035636
• Gene symbol: RIAM
• Gene ID (NCBI): 54518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.