RIBC2 Polyklonaler Antikörper

RIBC2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 15272-1-AP

Synonyme

C22orf11, RIB43A-like with coiled-coils protein 2

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  WB analysis using 15272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15272-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15272-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15272-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIBC2 antibody (15272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Zervixkarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

15272-1-AP bindet in WB, IHC, IF/ICC, ELISA RIBC2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RIBC2 fusion protein Ag7385
• Vollständiger Name: RIB43A domain with coiled-coils 2
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC003024
• Gene symbol: RIBC2
• Gene ID (NCBI): 26150
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIBC2 is a microtubule protein and is part of the double microtubules (DMTs) decorated with dynamic proteins in the ciliary axons. It is mainly located in the microtubules of cilia and is involved in flagellated sperm motility(PMID: 40265983).

Publikationen

Species	Application	Title
human	IHC	Front Mol Biosci; Potential biomarkers and immune infiltration linking endometriosis with recurrent pregnancy loss based on bioinformatics and machine learning; Authors - Jianhui Chen
Human		EMBO J; RNF26 binds perinuclear vimentin filaments to integrate ER and endolysosomal responses to proteotoxic stress; Authors - Tom Cremer