RIC8A Polyklonaler Antikörper

RIC8A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 18707-1-AP

Synonyme

Protein Ric-8A, RIC8, synembryn, Synembryn A, Synembryn-A

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  WB analysis of human brain using 18707-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18707-1-AP (RIC8A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18707-1-AP (RIC8A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18707-1-AP (RIC8A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 18707-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18707-1-AP (RIC8A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 18707-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIC8A antibody (18707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

18707-1-AP bindet in WB, IHC, IF/ICC, ELISA RIC8A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: resistance to inhibitors of cholinesterase 8 homolog A (C. elegans)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC011821
• Gene symbol: RIC8A
• Gene ID (NCBI): 60626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIC8A, also named as synembryn-A, is a Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins by exchanging bound GDP for free GTP. It is involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex. RIC8A also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation.

Publikationen

Species	Application	Title
mouse	IHC	Dev Dyn; Nucleotide exchange factor RIC-8 is indispensable in mammalian early development.; Authors - T?nissoo Tambet T