RICTOR Monoklonaler Antikörper

RICTOR Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D1C4

Kat-Nr. : 66867-2-PBS

Synonyme

1D1C4, AVO3 homolog, hAVO3, KIAA1999, Rapamycin-insensitive companion of mTOR

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  WB analysis using 66867-2-Ig (same clone as 66867-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66867-2-Ig (RICTOR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66867-2-Ig (same clone as 66867-2-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66867-2-Ig (same clone as 66867-2-PBS)

  Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66867-2-Ig (same clone as 66867-2-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 66867-2-Ig (same clone as 66867-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RICTOR antibody (66867-2-Ig, Clone: 1D1C4 ) at dilution of 1:1700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66867-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50234-1

  Cytometric bead array standard curve of MP50234-1, RICTOR Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66867-2-PBS. Detection antibody: 66867-3-PBS. Standard:Ag28487. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 66867-2-Ig

  Biolayer interferometry (BLl) kinetic assays of 66867-2-Ig against Human RICTOR were performed. The affinity constant is 146 nM.

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Geprüfte Anwendungen

Produktinformation

66867-2-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA RICTOR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RICTOR fusion protein Ag28487
• Vollständiger Name: rapamycin-insensitive companion of mTOR
• Berechnetes Molekulargewicht: 192 kDa
• Beobachtetes Molekulargewicht: 192 kDa
• GenBank-Zugangsnummer: BC029608
• Gene symbol: RICTOR
• Gene ID (NCBI): 253260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RICTOR, is a key component of the mTOR complex 2 (mTORC2) and is required for phosphorylation of Akt at serine 473. RICTOR is the upstream kinase of several AGC kinase family members including AKT, SGK, S6K mutants and several PKC isoforms. Activation of RICTOR-mTORC2 modifies actin organization and promotes cell proliferation and survival. Rictor is overexpressed in several cancers leading to hyperactive mTORC2 and has been shown to play a causal role in glioma formation. Rictor expression has been demonstrated to be regulated transcriptionally and via protein degradation.