RIG-1/DDX58 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RIG-1/DDX58 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20566-1-PBS

Synonyme

DDX58, RIG-I, Retinoic acid-inducible gene 1 protein, EC:3.6.4.13, DEAD box protein 58

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  WB analysis using 20566-1-AP (same clone as 20566-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20566-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 20566-1-AP (same clone as 20566-1-PBS)

  WB result of RIG-1/DDX58 antibody (20566-1-AP; 1:2000; room temperature for 1.5 hours) with wild-type and RIG-1/DDX58 knockout A431 cells. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 20566-1-AP (same clone as 20566-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20566-1-AP (same clone as 20566-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 20566-1-AP (same clone as 20566-1-PBS)

  IP result of anti-RIG-1/DDX58 (IP:20566-1-AP, 4ug; Detection:20566-1-AP 1:300) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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  IF Staining of H9C2 using 20566-1-AP (same clone as 20566-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using RIG-1/DDX58 antibody (20566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20566-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20566-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA RIG-1/DDX58 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 101~106 kDa
• GenBank-Zugangsnummer: NM_014314
• Gene symbol: RIG-1/DDX58
• Gene ID (NCBI): 23586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.