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RIG-1/DDX58 Monoklonaler Antikörper

RIG-1/DDX58 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3B10F5

Kat-Nr. : 67556-1-PBS

Synonyme

DDX58, RIG-I, 3B10F5, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58

Filter:
  • Western Blot (WB) analysis of various lysates using RIG-1/DDX58 Monoclonal antibody (67556-1-Ig)

    WB analysis using 67556-1-Ig (same clone as 67556-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67556-1-Ig (RIG-1/DDX58 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RIG-1/DDX58 Monoclonal antibody (67556-1-Ig)

    IF Staining of HepG2 using 67556-1-Ig (same clone as 67556-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RIG-1/DDX58 antibody (67556-1-Ig, Clone: 3B10F5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67556-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67556-1-PBS bindet in WB, IF/ICC, Indirect ELISA RIG-1/DDX58 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RIG-1/DDX58 fusion protein Ag18591
Vollständiger Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Berechnetes Molekulargewicht 925 aa, 106 kDa
Beobachtetes Molekulargewicht 101/106 kDa
GenBank-ZugangsnummerBC132786
Gene symbol RIG-1/DDX58
Gene ID (NCBI) 23586
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58,which has two isoform with the MW of 106 and 101 kDa.