RILP Rekombinanter Antikörper

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• KD/KO Validated

RILP Rekombinant Antikörper für WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230208G2

Kat-Nr. : 82996-4-PBS

Synonyme

230208G2, FLJ31193, PP10141, Rab-interacting lysosomal protein

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  WB analysis using 82996-4-RR (same clone as 82996-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82996-4-RR (RILP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 82996-4-RR (same clone as 82996-4-PBS)

  WB result of RILP antibody (82996-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RILP transfected HepG2 cells. This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82996-4-RR (same clone as 82996-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82996-4-RR (RILP antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82996-4-RR (same clone as 82996-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RILP Recombinant antibody (82996-4-RR, Clone:230208G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82996-4-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00025-1

  Cytometric bead array standard curve of MP00025-1, RILP Recombinant Matched Antibody Pair - PBS only. Capture antibody: 82996-4-PBS. Detection antibody: 82996-3-PBS. Standard: Ag4472. Range: 1.25-20 ng/mL.

• 

  Affinity and Kinetic Characterization of 82996-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82996-4-RR against Human IGFBP4 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82996-4-PBS bindet in WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA RILP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RILP fusion protein Ag4472
• Vollständiger Name: Rab interacting lysosomal protein
• Berechnetes Molekulargewicht: 401 aa, 41 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC031621
• Gene symbol: RILP
• Gene ID (NCBI): 83547
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.