RIOK3 Rekombinanter Antikörper

RIOK3 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242996A6

Kat-Nr. : 85449-5-RR

Synonyme

242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3

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  WB analysis using 85449-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85449-5-RR (RIOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 85449-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 85449-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 85449-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85449-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85449-5-RR against Human RIOK3 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen, A431-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85449-5-RR bindet in WB, IF/ICC, ELISA RIOK3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RIOK3 fusion protein Ag4497
• Vollständiger Name: RIO kinase 3 (yeast)
• Berechnetes Molekulargewicht: 516 aa, 60 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC039729
• Gene symbol: RIOK3
• Gene ID (NCBI): 8780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.