RIPK1-Specific Polyklonaler Antikörper

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• KD/KO Validated

RIPK1-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (3)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(139)

Rezensionen (1)

Kat-Nr. : 17519-1-AP

Synonyme

RIPK1, EC:2.7.11.1, Receptor interacting serine/threonine protein kinase 1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1

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• 

  WB analysis using 17519-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 17519-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 17519-1-AP (RIPK1-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse small intestine using 17519-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17519-1-AP (RIPK1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausmilzgewebe, Rattenherzgewebe, Rattenlebergewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 123 publications below
• IHC: See 16 publications below
• IF: See 21 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below

Produktinformation

17519-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA RIPK1-Specific und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hefe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: NM_003804
• Gene symbol: RIPK1
• Gene ID (NCBI): 8737
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis, and necroptosis and belongs to a novel class of kinases that function in cell survival and cell death mechanisms(PMID:22685397 ). It has 2 isoforms produced by alternative splicing. It also can exist as a protein with a lower molecular weight of about 25 kDa and 45 kDa in HAEC and HUVEC(PMID:22685397).

Publikationen

Species	Application	Title
mouse	WB	Acta Pharm Sin B; A new perspective of triptolide-associated hepatotoxicity: the relevance of NF- κ B and NF- κ B-mediated cellular FLICE-inhibitory protein.; Authors - Ziqiao Yuan
mouse	WB	Circ Res; AirnRegulates Igf2bp2 Translation in Cardiomyocytes.; Authors - Mohammed R Hosen
mouse	WB,IP	Nat Commun; Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis.; Authors - Jiefei Geng
mouse	IHC	Sci Total Environ; Evaluation of 6-PPD quinone toxicity on lung of male BALB/c mice by quantitative proteomics; Authors - Wenmiao He
mouse,rat	WB,IP,IF,CoIP	Sci Total Environ; Glycidol induces necroptosis and inflammation through autophagy-necrosome pathway in renal cell and mice; Authors - Jing Lu
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yuan (Verified Customer) (01-07-2020)

This RIPK-1 antibody was tried on the MCF7 tamoxifen resistant (5uM) cell line, the 74 kDa band was very faint, but the 45 kDa band is clear. In addition, some unspecific bands near 45 kDa showed up too.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MCF7-TR5