• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RIPK3 Monoklonaler Antikörper

RIPK3 Monoklonal Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F7B3

Kat-Nr. : 68786-2-PBS

Synonyme

RIP3, 1F7B3, EC:2.7.11.1, Receptor interacting protein 3, Receptor-interacting protein 3

Filter:
  • Western Blot (WB) analysis of various lysates using RIPK3 Monoclonal antibody (68786-2-Ig)

    WB analysis using 68786-2-Ig (same clone as 68786-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68786-2-Ig (RIPK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68786-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RIPK3 Monoclonal antibody (68786-2-Ig)

    WB analysis using 68786-2-Ig (same clone as 68786-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68786-2-Ig (RIPK3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68786-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50152-1

    Cytometric bead array standard curve of MP50152-1

    Cytometric bead array standard curve of MP50152-1, RIPK3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68786-1-PBS. Detection antibody: 68786-2-PBS. Standard:Ag11759. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP50152-3

    Cytometric bead array standard curve of MP50152-3

    Cytometric bead array standard curve of MP50152-3, RIPK3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68786-3-PBS. Detection antibody: 68786-2-PBS. Standard:Ag11759. Range: 0.391-50 ng/mL.

Geprüfte Anwendungen

Produktinformation

68786-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA RIPK3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen RIPK3 fusion protein Ag11759
Vollständiger Name receptor-interacting serine-threonine kinase 3
Berechnetes Molekulargewicht 518 aa, 57 kDa
Beobachtetes Molekulargewicht57 kDa
GenBank-ZugangsnummerBC062584
Gene symbol RIPK3
Gene ID (NCBI) 11035
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Receptor-interacting protein 3 (RIP3, also known as RIPK3) is a serine-threonine protein involved in the regulation of inflammatory signaling and cell death. RIPK3, also named as RIP3, a Ser/Thr kinase of RIP (Receptor Interacting Protein) family, is a nucleocytoplasmic shuttling protein and its unconventional nuclear localization signal (NLS, 442-472 aa) is sufficient to trigger apoptosis in the nucleus (PMID: 18533105). It has 3 isoforms produced by alternative splicing.

What is the molecular weight of RIP3? Is RIP3 post-translationally modified?

The molecular weight of RIP3 is 57 kDa. During the induction of necroptosis, RIP3 migrates slower in SDS-PAGE due to its phosphorylation (PMID: 19524512). Additionally, RIP3 can be a subject of poly-ubiquitination, when targeted for degradation.

Are there any splice isoforms of RIP3?

Apart from full-length RIP3, there are two reported splice isoforms of RIP3: RIP3β and RIP3γ, and 28 and 25 kDa, respectively (PMID: 15896315).

What is the subcellular localization of RIP3?

RIP3 can shuttle between the cytoplasm and the nucleus. Although RIP3 forms necrosomes in the cytoplasm, a recent study suggests that the phosphorylation of RIP3, required for necroptosis, may also occur in the nucleus (PMID: 30271893). Additionally, the induction of necrosis by reactive oxygen species can cause transient translocation of RIP3 to mitochondria (PMID: 25206339).

What is the role of RIP3 in cell death (necroptosis)?

Activation of RIP3 kinase is required for the induction of necroptosis (PMID: 19524512, 19524513 and 19498109). Activation of RIP3 can be induced by interferons, death ligands, or by Toll-like receptors in response to pathogens. That leads to the phosphorylation of RIP3 and the formation of a β-amyloid-like protein complex. Phosphorylated RIP3 acts downstream by phosphorylation of MLKL (PMID: 30131615).

How to study necroptosis in a cell-based system?

The choice of the cell line is important. Many commonly used immortalized cell types are derived from cancers and may have very low RIL3 expression level (PMID: 25952668). Those cell lines are not going to be responsive to necrotic stimuli. A few of the examined cell types have high RIP3 levels: Jurkat, CCRF-CEM, U937, L929 cells, and mouse embryonic fibroblasts (PMID: 19524512). Good necroptosis readouts reflect an increased level of RIP3 protein and its phosphorylation.