RIT1 Rekombinanter Antikörper

RIT1 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242593E4

Kat-Nr. : 85347-1-PBS

Synonyme

RIBB, Ras like without CAAX protein 1, Ras like without CAAX 1, Ras like protein expressed in many tissues, GTP binding protein Rit1

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  WB analysis using 85347-1-RR (same clone as 85347-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 85347-1-RR (same clone as 85347-1-PBS)

  Mouse kidney tissue was subjected to SDS PAGE followed by western blot with 85347-1-RR (RIT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85347-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85347-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85347-1-RR against Human RIT1 were performed. The affinity constant is 0.505 nM.

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Geprüfte Anwendungen

Produktinformation

85347-1-PBS bindet in WB, Indirect ELISA RIT1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RIT1 fusion protein Ag25636
• Vollständiger Name: Ras-like without CAAX 1
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC104186
• Gene symbol: RIT1
• Gene ID (NCBI): 6016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIT1 (Ras-like without CAAX protein 1) is also named as RIBB, ROC1 and GTP-binding protein Rit1. RIT1 serves as a regulatory factor for neuronal cell proliferation, survival, and differentiation (PMID: 28007959). Rit1 mediates oxidative stress resistance, contributing to cell survival via the p38 MAPK signaling pathway (PMID: 21737674). As a small G protein of Ras family, RIT1 plays a critical role in various tumors (such as hepatocellular carcinoma, HCC) (PMID: 38017479). It is worth noting that RIT1 is frequently amplified in various human cancers including HCC, lung adenocarcinoma, cholangiocarcinoma, uterine carcinosarcoma, breast cancer, and ovarian cancer (PMID: 38017479).