RIT2 Polyklonaler Antikörper

RIT2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12473-1-PBS

Synonyme

---

• 

  WB analysis using 12473-1-AP (same clone as 12473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12473-1-AP (RIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

  IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with mouse brain tissue lysate 1800 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat brain using 12473-1-AP (same clone as 12473-1-PBS)

  IP result of anti-RIT2 (IP:12473-1-AP, 4ug; Detection:12473-1-AP 1:1000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12473-1-AP (same clone as 12473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 12473-1-AP (same clone as 12473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12473-1-AP (RIT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12473-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12473-1-PBS bindet in WB, IHC, IP, Indirect ELISA RIT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RIT2 fusion protein Ag3160
• Vollständiger Name: Ras-like without CAAX 2
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC018060
• Gene symbol: RIT2
• Gene ID (NCBI): 6014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).