RIT2 Rekombinanter Antikörper

RIT2 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241916A7

Kat-Nr. : 84520-1-PBS

Synonyme

241916A7, EC:3.6.5.2, GTP binding protein Rit2, GTP-binding protein Rit2, Ras like protein expressed in neurons

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  WB analysis using 84520-1-RR (same clone as 84520-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84520-1-RR (RIT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84520-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84520-1-RR (same clone as 84520-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84520-1-RR (RIT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84520-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01369-1

  Cytometric bead array standard curve of MP01369-1, RIT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84520-2-PBS. Detection antibody: 84520-1-PBS. Standard: Ag3160. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84520-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84520-1-RR against Human RIT2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84520-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA RIT2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RIT2 fusion protein Ag3160
• Vollständiger Name: Ras-like without CAAX 2
• Berechnetes Molekulargewicht: 217 aa, 25 kDa
• Beobachtetes Molekulargewicht: 25-28 kDa
• GenBank-Zugangsnummer: BC018060
• Gene symbol: RIT2
• Gene ID (NCBI): 6014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIT2 (AKA: Rin, Ras-like in neurons) is a small, neuronal, ras-like GTPase with enriched expression in SNc DANs (PMID: 38395968). RIT2 is a member of the Ras superfamily that plays important roles in many vital cellular functions, such as differentiation and survival (PMID: 29860660). Rit2 directly interacts with the DA transporter (DAT), and is required for regulated DAT membrane trafficking. In cell culture models, Rit2 is required for EGF- and NGF-mediated neurite outgrowth, NGF-mediated ERK phosphorylation, and cell viability (PMID: 38395968).