RMND5A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RMND5A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 17559-1-AP

Synonyme

RMD5, Protein RMD5 homolog A, P44CTLH, EC:2.3.2.27, E3 ubiquitin-protein transferase RMND5A

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  WB analysis using 17559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17559-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

17559-1-AP bindet in WB, IHC, ELISA RMND5A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RMND5A fusion protein Ag11751
• Vollständiger Name: required for meiotic nuclear division 5 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 391 aa, 44 kDa
• Beobachtetes Molekulargewicht: 40-44 kDa
• GenBank-Zugangsnummer: BC047668
• Gene symbol: RMND5A
• Gene ID (NCBI): 64795
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cancers (Basel); Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.; Authors - Chunyuan Tang ; KD Validated
human	WB	Chembiochem; Identifying E3 ligase substrates with quantitative degradation proteomics; Authors - Victoria N Jordan
human	WB	Mol Cell; mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway; Authors - Sang Ah Yi ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

H (Verified Customer) (09-27-2020)

I used this antibody to screen CRISPR-knock out, and found it difficult to do so (at least in HEK293T cells). There are so so many non-specific bands, and the right MW band was weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

RMND5A Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:17559-1-AP)