RNASE4 Polyklonaler Antikörper

RNASE4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30732-1-AP

Synonyme

Ribonuclease 4, RNase 4, RNASE4, RNS4

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  WB analysis using 30732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 30732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of rat lung using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse bladder using 30732-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human milk, THP-1-Zellen
• Erfolgreiche Detektion in IHC: Rattenlungengewebe, Maus-Blasengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30732-1-AP bindet in WB, IHC, ELISA RNASE4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASE4 fusion protein Ag29272
• Vollständiger Name: ribonuclease, RNase A family, 4
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC015520
• Gene symbol: RNASE4
• Gene ID (NCBI): 6038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human Ribonuclease 4 (RNase 4), one of the eight members of the human RNase A superfamily of endoribonucleases, is a uridine-specific endoribonuclease with a preference to cleave RNA after uridine residues prior to purines (PMID: 33818125). RNase 4 is a newly identified host defense peptide in the human kidney and bladder and kills uropathogenic E. coli (UPEC) and multi-drug resistant UPEC (PMID: 28955965).