RNASEH1 Rekombinanter Antikörper

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• KD/KO Validated

RNASEH1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C12

Kat-Nr. : 82771-1-PBS

Synonyme

RNH1, RNASEH 1, RNase H1, EC:3.1.26.4, 2C12

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  WB analysis using 82771-1-RR (same clone as 82771-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

  WB result of RNASEH1 antibody (82771-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNASEH1 transfected HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82771-1-RR (same clone as 82771-1-PBS)

  WB result of RNASEH1 antibody (82771-1-RR; 1:15000; room temperature for 1.5 hours) with wild-type and RNASEH1 knockout HeLa cells. This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82771-1-RR (same clone as 82771-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82771-1-RR (RNASEH1 antibody) at dilution of 1:3000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82771-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82771-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82771-1-RR against Human RNASEH1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82771-1-PBS bindet in WB, IHC, Indirect ELISA RNASEH1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNASEH1 fusion protein Ag7990
• Vollständiger Name: ribonuclease H1
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC002973
• Gene symbol: RNASEH1
• Gene ID (NCBI): 246243
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].