RNASEH2A Polyklonaler Antikörper

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• KD/KO Validated

RNASEH2A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16132-1-AP

Synonyme

AGS4, Aicardi-Goutieres syndrome 4 protein, EC:3.1.26.4, JUNB, Ribonuclease H2 subunit A

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  WB analysis using 16132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16132-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16132-1-AP (RNASEH2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Lebergewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Produktinformation

16132-1-AP bindet in WB, IHC, ELISA RNASEH2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASEH2A fusion protein Ag9118
• Vollständiger Name: ribonuclease H2, subunit A
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC011748
• Gene symbol: RNASEH2A
• Gene ID (NCBI): 10535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Publikationen

Species	Application	Title
human	WB	Commun Biol; RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells; Authors - Sho Sugawara
human	WB	PLoS Genet; Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.; Authors - Sarah K Deasy ; KD Validated
		Oncol Rep; RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis.; Authors - Bin Dai
human	IHC	Tumori; Hypoxia-induced RNASEH2A limits activation of cGAS-STING signaling in HCC and predicts poor prognosis.; Authors - Fengbo Zhao
human	WB,IHC	Cancer Res Commun; Ribonuclease H2 Subunit A Preserves Genomic Integrity and Promotes Prostate Cancer Progression; Authors - Naoki Kimura ; KD Validated
mouse,human	WB	bioRxiv; RTF2 controls replication repriming and ribonucleotide excision at the replisome; Authors - Brooke A Conti