• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RNASEH2B Rekombinanter Antikörper

RNASEH2B Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241131F1

Kat-Nr. : 84097-6-PBS

Synonyme

ribonuclease H2, subunit B, Ribonuclease H2 subunit B, DLEU8, Aicardi-Goutieres syndrome 2 protein, AGS2

Filter:
  • Western Blot (WB) analysis of various lysates using RNASEH2B Recombinant antibody (84097-6-RR)

    WB analysis using 84097-6-RR (same clone as 84097-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84097-6-RR (RNASEH2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNASEH2B Recombinant antibody (84097-6-RR)

    IF Staining of HeLa using 84097-6-RR (same clone as 84097-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNASEH2B antibody (84097-6-RR, Clone: 241131F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84097-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84097-6-RR

    Affinity and Kinetic Characterization of 84097-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84067-4-RR against Human RNASEH2B were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

84097-6-PBS bindet in WB, IF/ICC, Indirect ELISA RNASEH2B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RNASEH2B fusion protein Ag33669
Vollständiger Name ribonuclease H2, subunit B
Berechnetes Molekulargewicht 331 aa, 37 kDa
Beobachtetes Molekulargewicht35 kDa
GenBank-ZugangsnummerBC036744
Gene symbol RNASEH2B
Gene ID (NCBI) 79621
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ribonuclease H2 is the major nuclear enzyme degrading cellular RNA/DNA hybrids in eukaryotes and the sole nuclease known to be able to hydrolyze ribonucleotides misincorporated during genomic replication. RNASEH2B and RNASEH2C are the non-catalytic subunits of RNase H2 complex. Defects in RNASEH2B are a cause of Aicardi-Goutieres syndrome type 2 (AGS2), a genetically heterogeneous disease with clinical features as thrombocytopenia, hepatosplenomegaly and elevated hepatic transaminases along with intermittent fever may erroneously suggest an infective process.