RNASEL Polyklonaler Antikörper

RNASEL Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 22577-1-AP

Synonyme

2 5A dependent ribonuclease, 2 5A dependent RNase, PRCA1, Ribonuclease 4, Ribonuclease L, RNase L, RNASEL, RNS4

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Galerie der Validierungsdaten

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  WB analysis using 22577-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 22577-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22577-1-AP (RNASEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 22577-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22577-1-AP (RNASEL Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 22577-1-AP

  Immunofluorescent analysis of HepG2 cells using 22577-1-AP (RNASEL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

22577-1-AP bindet in WB, IHC, IF, ELISA RNASEL und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNASEL fusion protein Ag17409
• Vollständiger Name: ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent)
• Berechnetes Molekulargewicht: 741 aa, 84 kDa
• Beobachtetes Molekulargewicht: 84 kDa; 73 kDa
• GenBank-Zugangsnummer: BC115697
• Gene symbol: RNASEL
• Gene ID (NCBI): 6041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.

Publikationen

Species	Application	Title
human	wb	Adv Sci (Weinh); The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells.; Authors - Qixue Wang
human	WB	Virol Sin; IFN-β1b induces OAS3 to inhibit EV71 via IFN-β1b/JAK/STAT1 pathway; Authors - Baisong Zheng
mouse,rat	IF	Apoptosis; The function of RNase L and its degradation mechanism in cardiac acute ischemic injury.; Authors - Qiang Ma
		Cardiovasc Diabetol; Human serum RNase-L level is inversely associated with metabolic syndrome and age.; Authors - Yi-Ting Wang
mouse	WB	iScience; Inhibition of cGAS-Mediated Interferon Response Facilitates Transgene Expression.; Authors - Yajuan Fu
mouse	WB	Cell Rep; Deficiency of ER Ca2+ sensor STIM1 in AgRP neurons confers protection against dietary obesity.; Authors - Zhuo Chen