RNASET2 Rekombinanter Antikörper

RNASET2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240581C4

Kat-Nr. : 83650-1-RR

Synonyme

RNase T2, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19, bA514O12.3

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  WB analysis using 83650-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83650-1-RR (RNASET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83650-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83650-1-RR against Human RNASET2 were performed. The affinity constant is 2.09 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83650-1-RR bindet in WB, ELISA RNASET2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNASET2 fusion protein Ag28353
• Vollständiger Name: ribonuclease T2
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 25-45 kDa
• GenBank-Zugangsnummer: BC039713
• Gene symbol: RNase T2
• Gene ID (NCBI): 8635
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).