RNF10 Polyklonaler Antikörper

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• KD/KO Validated

RNF10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16936-1-AP

Synonyme

RING finger protein 10, RIE2, KIAA0262, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF10

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16936-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16936-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16936-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16936-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16936-1-AP (RNF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 16936-1-AP

  IP result of anti-RNF10 (IP:16936-1-AP, 4ug; Detection:16936-1-AP 1:500) with PC-3 cells lysate 1480 ug.

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16936-1-AP (RNF10 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16936-1-AP (RNF10 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16936-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16936-1-AP (RNF10 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16936-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF10 antibody (16936-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, humanes Hirngewebe, Jurkat-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16936-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RNF10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF10 fusion protein Ag10433
• Vollständiger Name: ring finger protein 10
• Berechnetes Molekulargewicht: 811 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC016622
• Gene symbol: RNF10
• Gene ID (NCBI): 9921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.

Publikationen

Species	Application	Title
human	WB,IF	Elife; Ring finger protein 10 is a novel synaptonuclear messenger encoding activation of NMDA receptors in hippocampus.; Authors - Margarita C Dinamarca ; KD Validated
human	WB	Cell Rep; The E3 ubiquitin ligase RNF10 modifies 40S ribosomal subunits of ribosomes compromised in translation.; Authors - Aitor Garzia ; KO Validated
mouse,human	WB	Nat Cell Biol; Gut microbial metabolite facilitates colorectal cancer development via ferroptosis inhibition; Authors - Weiwei Cui
mouse		J Clin Invest; RNF4 sustains Myc-driven tumorigenesis by facilitating DNA replication; Authors - Joonyoung Her
human	WB	Nat Commun; E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance; Authors - Janina A Lehmann
human	WB	Mol Cell; The integrated stress response regulates 18S nonfunctional rRNA decay in mammals; Authors - Aaztli R Coria ; KO Validated