RNF10 Rekombinanter Antikörper

RNF10 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250438C11

Kat-Nr. : 86019-1-PBS

Synonyme

E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10

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  WB analysis of Jurkat using 86019-1-RR (same clone as 86019-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 86019-1-RR (same clone as 86019-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 86019-1-RR (same clone as 86019-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  IF Staining of K562 using 86019-1-RR (same clone as 86019-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K562 using RNF10 antibody (86019-1-RR, Clone: 250438C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86019-1-PBS bindet in WB, IF/ICC, Indirect ELISA RNF10 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF10 fusion protein Ag10433
• Vollständiger Name: ring finger protein 10
• Berechnetes Molekulargewicht: 811 aa, 90 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: BC016622
• Gene symbol: RNF10
• Gene ID (NCBI): 9921
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.