RNF126 Monoklonaler Antikörper

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• KD/KO Validated

RNF126 Monoklonal Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1E1B10

Kat-Nr. : 66647-1-Ig

Synonyme

1E1B10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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  WB analysis of Jurkat using 66647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66647-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66647-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 66647-1-Ig

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Monoclonal antibody (66647-1-Ig, Clone:1E1B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Ramos-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in FC (Intra): Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

66647-1-Ig bindet in WB, IHC, IF, FC (Intra), ELISA RNF126 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RNF126 fusion protein Ag13816
• Vollständiger Name: ring finger protein 126
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC001442
• Gene symbol: RNF126
• Gene ID (NCBI): 55658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu ; KD Validated
human	WB	Exp Eye Res; Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.; Authors - Jing Zhang
human	WB	Cell Death Dis; ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.; Authors - Shengjun Geng
human	WB	Cell Chem Biol; Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway; Authors - Yali Ci
human	IHC,IF	J Cell Physiol; ZNF263 cooperates with ZNF31 to promote the drug resistance and EMT of pancreatic cancer through transactivating RNF126; Authors - Jiawei Zhang
human	WB	ACS Cent Sci; DCAF16-Based Covalent Handle for the Rational Design of Monovalent Degraders; Authors - Melissa Lim ; KO Validated