RNF126 Monoklonaler Antikörper

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• KD/KO Validated

RNF126 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1E1B10

Kat-Nr. : 66647-1-Ig

Synonyme

ring finger protein 126, RNF126

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 66647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66647-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66647-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, K-562-Zellen, Ramos-Zellen, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

66647-1-Ig bindet in WB, ELISA RNF126 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RNF126 fusion protein Ag13816
• Vollständiger Name: ring finger protein 126
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC001442
• Gene symbol: RNF126
• Gene ID (NCBI): 55658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).

Publikationen

Species	Application	Title
human	WB	Mol Cell; RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.; Authors - Xianyan Hu ; KD Validated
human	WB	Exp Eye Res; Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.; Authors - Jing Zhang
human	WB	Cell Death Dis; ARIH2 regulates the proliferation, DNA damage and chemosensitivity of gastric cancer cells by reducing the stability of p21 via ubiquitination.; Authors - Shengjun Geng
human	WB	Cell Chem Biol; Bortezomib inhibits ZIKV/DENV by interfering with viral polyprotein cleavage via the ERAD pathway; Authors - Yali Ci