RNF126 Monoklonaler Antikörper

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RNF126 Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E1B10

Kat-Nr. : 66647-1-PBS

Synonyme

1E1B10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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  WB analysis of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66647-1-Ig (same clone as 66647-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66647-1-Ig (same clone as 66647-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66647-1-Ig (RNF126 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 66647-1-Ig (same clone as 66647-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Monoclonal antibody (66647-1-Ig, Clone:1E1B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66647-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66647-1-PBS bindet in WB, FC (Intra), Indirect ELISA RNF126 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RNF126 fusion protein Ag13816
• Vollständiger Name: ring finger protein 126
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36-38 kDa
• GenBank-Zugangsnummer: BC001442
• Gene symbol: RNF126
• Gene ID (NCBI): 55658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).